3GHP
Structure of the second type II cohesin module from the adaptor ScaA scaffoldin of Acetivibrio cellulolyticus (including long C-terminal linker)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9393 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 68.759, 159.221, 44.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.601 - 2.487 |
R-factor | 0.217 |
Rwork | 0.212 |
R-free | 0.31700 |
Starting model (for MR) | 3fnk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.481 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.487 | 2.487 |
Rmerge | 0.073 | 0.377 |
Number of reflections | 12802 | |
<I/σ(I)> | 20.7 | 2.1 |
Completeness [%] | 71.9 | 24.4 |
Redundancy | 4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 10%(v/v) 2-propanol, 17%(w/v) PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |