3GHN
Crystal structure of the exosite-containing fragment of human ADAMTS13 (form-2)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2008-02-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 138.577, 51.370, 76.371 |
Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
Resolution | 29.090 - 2.800 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.28000 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.062 | 0.403 |
Number of reflections | 12811 | |
<I/σ(I)> | 13.3 | |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 26% PEG1500, 100mM MES/NaOH, 8% pentaerythritol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |