3GGR

Crystal Structure of the Human Rad9-Hus1-Rad1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00716
Spacegroup name	P 1 21 1
Unit cell lengths	71.029, 67.164, 83.405
Unit cell angles	90.00, 97.58, 90.00

Refinement procedure

Resolution	15.000 - 3.200
R-factor	0.291
Rwork	0.289
R-free	0.30600
Structure solution method	SIRAS
RMSD bond length	0.011
RMSD bond angle	1.728
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.280
High resolution limit [Å]	3.200	3.200
Rmerge	0.080	0.432
Number of reflections	13010
<I/σ(I)>		4.2
Completeness [%]	99.8	99.5
Redundancy	6.5	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	8% PEG 2000 MME, 100mM Tris-HCl,200mM Trimethylamine N-oxide dihydrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	8	289	8% PEG 2000 MME, 100mM Tris-HCl,200mM Trimethylamine N-oxide dihydrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K