3GGQ

Dimerization of Hepatitis E Virus Capsid Protein E2s Domain is Essential for Virus-Host Interaction

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-27
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9206,0.9208
Spacegroup name	H 3 2
Unit cell lengths	111.448, 111.448, 84.333
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.000
R-factor	0.215
Rwork	0.198
R-free	0.24010
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.054
Number of reflections	24473
Completeness [%]	92.6	68.2
Redundancy	5.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	0.1M HEPES, pH7.5, 12% PEG3350, 5mM cobalt chloride hexahydrate, 5mM nickel (II) chloride hexahydrate, 5mM cadmium chloride dehydrate, 5mM magnesium chloride hexahydrate, VAPOR DIFFUSION, HANGING DROP, temperature 294K