3GG2
Crystal structure of UDP-glucose 6-dehydrogenase from Porphyromonas gingivalis bound to product UDP-glucuronate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.365, 143.489, 85.501 |
| Unit cell angles | 90.00, 112.46, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.531 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.368 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.117 | 0.412 |
| Total number of observations | 137432 | |
| Number of reflections | 188163 | |
| <I/σ(I)> | 8.1 | 2.9 |
| Completeness [%] | 93.7 | 96.1 |
| Redundancy | 4.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.4 | 294 | 100mM Bis-Tris pH 5.4, 21% PEG 3350, 50mM magnesium chloride, vapor diffusion, temperature 294K |
