3GFB
L-Threonine Dehydrogenase (TkTDH) from the hyperthermophilic archaeon Thermococcus kodakaraensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-12 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 124.481, 124.481, 271.143 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.920 - 2.400 |
| R-factor | 0.22039 |
| Rwork | 0.217 |
| R-free | 0.27659 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dfv |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.772 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.920 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.146 | 0.590 |
| Number of reflections | 84056 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.05 M sodium citrate, 2.4 M ammonium sulfate, 0.1 M sodium/potassium tartrate, 5%(v/v) glycerol and 0.1 mM zinc chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
