3GEW
FaeE-FaeG chaperone-major pilin complex of F4 ad fimbriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.576, 89.757, 85.826 |
| Unit cell angles | 90.00, 113.94, 90.00 |
Refinement procedure
| Resolution | 17.890 - 2.000 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3F65 AND 2J6G |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.157 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.105 | 0.530 |
| Number of reflections | 68837 | |
| <I/σ(I)> | 10.91 | 2.98 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 283 | 2M (NH4)2SO4, 0.1M Na acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
