3GEI
Crystal structure of MnmE from Chlorobium tepidum in complex with GCP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9796 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 139.882, 224.572, 156.788 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.940 - 3.400 |
R-factor | 0.24624 |
Rwork | 0.245 |
R-free | 0.26891 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xzp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.198 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.425 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.129 | 0.654 |
Number of reflections | 34042 | |
<I/σ(I)> | 12.13 | 2.02 |
Completeness [%] | 99.2 | 100 |
Redundancy | 7.45 | 7.61 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100mM MES, 46mM NaOH, 12% PEG 4000, 40mM NaCl, 5mM GCP, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |