3GEF

Crystal structure of the R482W mutant of lamin A/C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.09
Spacegroup name	P 21 21 21
Unit cell lengths	62.195, 84.035, 98.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 1.500
Rwork	0.214
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ifr
RMSD bond length	0.018
RMSD bond angle	2.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	1.500
Rmerge	0.062
Number of reflections	68652
<I/σ(I)>	31.2
Completeness [%]	82.2
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	298	0.9M sodium citrate, 20mM DTT, 0.1M sodium cacodylate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K