3GE9
A Structurally Atypical ThyX from Corynebacterium glutamicum NCHU 87078 Is Not Required for Thymidylate Biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 96.322, 96.322, 64.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.500 - 2.610 |
| R-factor | 0.23439 |
| Rwork | 0.232 |
| R-free | 0.28411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.689 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.500 | |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.388 |
| Number of reflections | 9629 | |
| <I/σ(I)> | 28.7 | |
| Completeness [%] | 99.0 | 99.2 |
| Redundancy | 9.1 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
