3GCZ
Yokose virus Methyltransferase in complex with AdoMet
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 32 |
| Unit cell lengths | 68.912, 68.912, 50.806 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.16863 |
| Rwork | 0.167 |
| R-free | 0.19810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r6a |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.367 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 29342 | |
| <I/σ(I)> | 24.6 | 3.1 |
| Completeness [%] | 98.7 | 91.1 |
| Redundancy | 10.9 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2% PEG 400, 1.5M ammonium sulphate, 100mM HEPES-Na pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
