3GCV
Human P38 MAP Kinase in Complex with RL62
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-08 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54170 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.785, 70.003, 74.513 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.880 - 2.300 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.472 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.256 |
| Number of reflections | 16273 | 1930 |
| <I/σ(I)> | 17.06 | 6.3 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.63 | 4.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 100 mM MES, 20-30% PEG4000, 50 mM n-BOG, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
