3GC3

Crystal Structure of Arrestin2S and Clathrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-01
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	72.857, 126.173, 129.505
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.070 - 2.200
R-factor	0.203
Rwork	0.200
R-free	0.25100
Structure solution method	MIR
Starting model (for MR)	1g4m
RMSD bond length	0.031
RMSD bond angle	2.633
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.4.0034)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.200
Rmerge	0.056
Number of reflections	61963
<I/σ(I)>	21
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	100 mM bis_tris propane 4 -4.5 M ammonium acetate 4.5% ethylene glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K