3GBI
The Rational Design and Structural Analysis of a Self-Assembled Three-Dimensional DNA Crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2008-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 107.161, 107.161, 93.144 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.288 - 4.018 |
| R-factor | 0.246 |
| Rwork | 0.240 |
| R-free | 0.30900 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.364 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL2Map |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.300 | 4.070 |
| High resolution limit [Å] | 4.010 | 4.000 |
| Rmerge | 0.067 | |
| Number of reflections | 3211 | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 96.1 | 100 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Grown by vapor diffusion while treated with a controlled temperature gradient from 333 degs to 293 degs., pH 8.5, VAPOR DIFFUSION, SITTING DROP |
