3GAX
Crystal structure of monomeric human cystatin C stabilized against aggregation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 61 |
| Unit cell lengths | 76.260, 76.260, 97.576 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.260 - 1.700 |
| R-factor | 0.13926 |
| Rwork | 0.138 |
| R-free | 0.16720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g96 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.746 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.896 |
| Number of reflections | 35237 | |
| <I/σ(I)> | 25.9 | 2 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 10.5 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 1.25M Ammonium phosphate, 150mM Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
