3GA3
Crystal structure of the C-terminal domain of human MDA5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.410, 60.920, 36.443 |
| Unit cell angles | 90.00, 102.58, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.450 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RIG-I CTD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.560 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.039 | 0.064 |
| Number of reflections | 20278 | |
| <I/σ(I)> | 64.5 | 45.6 |
| Completeness [%] | 94.2 | 86.8 |
| Redundancy | 4.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 277 | 0.1 M Tris, 14 to 18% ethanol, pH 8.5, EVAPORATION, temperature 277K |
