3GA2
Crystal structure of the Endonuclease_V (BSU36170) from Bacillus subtilis, Northeast Structural Genomics Consortium Target SR624
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97902 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 61.806, 83.861, 52.287 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.100 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.24300 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB (then SOLVE/RESOLVE) |
Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.095 | 0.353 |
Number of reflections | 30177 | |
<I/σ(I)> | 20.1 | 3.8 |
Completeness [%] | 98.2 | 88.2 |
Redundancy | 7.2 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, and 5 mM DTT. Reservoir solution: 16% PEG 3350 and 200 mM ammonium tartrate., VAPOR DIFFUSION, HANGING DROP, temperature 291K |