3G9W

Crystal Structure of Talin2 F2-F3 in Complex with the Integrin Beta1D Cytoplasmic Tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 21 21 21
Unit cell lengths	53.260, 108.720, 131.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.941 - 2.165
R-factor	0.2148
Rwork	0.213
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1MIX 1mk7 1mk9
RMSD bond length	0.005
RMSD bond angle	0.874
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.950	2.280
High resolution limit [Å]	2.165	2.165
Rmerge	0.114	0.338
Number of reflections	41362
<I/σ(I)>	8.3	3.5
Completeness [%]	99.6	99.9
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1M ammonium acetate, 0.02M magnesium chloride, 0.05M HEPES, 5% PEG 8k, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K