3G9J
GR DNA binding domain:Pal, 18bp complex-36
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-25 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 1.115878 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.069, 102.096, 112.076 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.125 - 2.320 |
| R-factor | 0.1884 |
| Rwork | 0.184 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1glu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.225 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.079 | 0.615 |
| Number of reflections | 20142 | |
| <I/σ(I)> | 23.1 | 3.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.5 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 50mM HEPES, pH 7.0, 100mM KCl, 10mM CaCl2, 10% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 2 | HEPES | ||
| 5 | 1 | 2 | KCl | ||
| 6 | 1 | 2 | CaCl2 | ||
| 7 | 1 | 2 | PEG400 |
