3G91
1.2 Angstrom structure of the exonuclease III homologue Mth0212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.330, 72.110, 46.330 |
| Unit cell angles | 90.00, 117.96, 90.00 |
Refinement procedure
| Resolution | 9.990 - 1.230 |
| R-factor | 0.1222 |
| R-free | 0.17180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fzi |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.031 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.270 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.029 | 0.131 |
| Number of reflections | 73200 | 6297 |
| <I/σ(I)> | 28.1 | 9 |
| Completeness [%] | 97.9 | 92.7 |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | reservoir solution: 20% (v/v) MPD, 40mM MgAc, 50mM sodium cacodylate pH 6.0; protein solution: 180mM KCl, 10mM KH2PO4/K2HPO4 pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
