3G8T
Crystal structure of the G33A mutant Bacillus anthracis glmS ribozyme bound to GlcN6P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.389, 232.671, 106.576 |
| Unit cell angles | 90.00, 92.21, 90.00 |
Refinement procedure
| Resolution | 28.230 - 3.000 |
| R-factor | 0.253 |
| Rwork | 0.250 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.892 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.230 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.120 | 0.046 | |
| Number of reflections | 46457 | ||
| <I/σ(I)> | 9.863 | ||
| Completeness [%] | 97.6 | 98.2 | 94.6 |
| Redundancy | 4.1 | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion, sitting drops | 6.8 | 298 | 11% PEG 8000, 9% DMSO, 0.02M SODIUM CACODYLATE, 0.02M MAGNESIUM CHLORIDE, 0.15M POTASSIUM CHLORIDE, pH 6.8, vapor diffusion, sitting drops, temperature 298K |
