3G7Z
CcdB dimer in complex with two C-terminal CcdA domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.248, 38.377, 62.498 |
| Unit cell angles | 90.00, 94.68, 90.00 |
Refinement procedure
| Resolution | 32.674 - 2.351 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ZOR chains A B |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.040 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.674 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.142 | 0.297 |
| Number of reflections | 9426 | |
| <I/σ(I)> | 7.7 | 2.9 |
| Completeness [%] | 80.9 | 64.6 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M LiSO4, 0.1 M Tris, 30% PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
