3G7Y

Crystal structure of oxidized Ost6L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-03-05
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	58.031, 59.391, 105.866
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.310 - 2.215
R-factor	0.2258
Rwork	0.219
R-free	0.27960
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	Ost6L structure solved by SAD (bromide signal)
RMSD bond length	0.005
RMSD bond angle	0.908
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.310	2.300
High resolution limit [Å]	2.210	2.210
Number of reflections	9389
<I/σ(I)>	18	5.9
Completeness [%]	98.9	92
Redundancy	7	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	18% PEG 3350, 50 mM Na-citrate, pH 3.0, protein solution buffer: 50 mM Tris-HCl, pH 8.0 mixing ratio 1.5(protein):1(precipitant), VAPOR DIFFUSION, SITTING DROP, temperature 277K