3G7W

Islet Amyloid Polypeptide (IAPP or Amylin) Residues 1 to 22 fused to Maltose Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98085
Spacegroup name	P 41 21 2
Unit cell lengths	84.495, 84.495, 144.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.220 - 1.750
R-factor	0.171
Rwork	0.170
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mpd
RMSD bond length	0.009
RMSD bond angle	1.193
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	90.000	1.890
High resolution limit [Å]	1.750	2.990	1.750
Rmerge	0.099	0.097	0.431
Number of reflections	53547
<I/σ(I)>	16.958
Completeness [%]	99.8	99	100
Redundancy	12.3	11.8	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	2.0 M Ammonium Sulfate, 0.1M Sodium Acetate pH 4.6, vapor diffusion, hanging drop, temperature 298K