3G7G
Crystal structure of the protein with unknown function from Clostridium acetobutylicum ATCC 824
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.048, 166.841, 66.974 |
| Unit cell angles | 90.00, 119.06, 90.00 |
Refinement procedure
| Resolution | 58.620 - 1.990 |
| R-factor | 0.19649 |
| Rwork | 0.193 |
| R-free | 0.25370 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.648 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.620 | 2.050 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.103 | 0.439 |
| Number of reflections | 81173 | |
| <I/σ(I)> | 12.63 | 1.47 |
| Completeness [%] | 97.9 | 84.82 |
| Redundancy | 4.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 20% PEG 3350, 0.1M Bis-tris, 0.2M NaCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
