3G78

Insight into group II intron catalysis from revised crystal structure

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	89.109, 94.970, 225.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.290 - 2.800
R-factor	0.19605
Rwork	0.194
R-free	0.22661
RMSD bond length	0.005
RMSD bond angle	1.210
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	REFMAC (5.4.0077)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.072
Number of reflections	47323
<I/σ(I)>	20.71	0.37
Completeness [%]	98.9	92.1
Redundancy	6.3	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1