3G73
Structure of the FOXM1 DNA binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-01 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 1.0723 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.070, 119.750, 152.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.940 - 2.210 |
| R-factor | 0.20557 |
| Rwork | 0.204 |
| R-free | 0.23425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c6y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.446 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.058 | 0.450 |
| Number of reflections | 27165 | |
| <I/σ(I)> | 16.1 | 3.1 |
| Completeness [%] | 91.4 | 59.4 |
| Redundancy | 5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 24% PEG3350, 0.2M Sodium Malonate, pH7.5, 3uL Protein at 12g/L mixed with DNA at 0.5mM added tO 3uL of reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG3350 | ||
| 2 | 1 | 1 | Sodium Malonate | ||
| 3 | 1 | 1 | HOH | ||
| 4 | 1 | 2 | PEG3350 | ||
| 5 | 1 | 2 | Sodium Malonate | ||
| 6 | 1 | 2 | HOH |
