3G6P
GR DNA binding domain:FKBP5 complex, 18bp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-10 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 1.115894 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.060, 100.110, 112.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.694 - 1.985 |
| R-factor | 0.2155 |
| Rwork | 0.214 |
| R-free | 0.23890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1glu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.233 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.079 | 0.031 |
| Number of reflections | 30730 | |
| <I/σ(I)> | 14.03 | 0.38 |
| Completeness [%] | 98.0 | 83.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 50mM Na cacodylate pH 6.0, 1mM Spermidine, 20mM MgCL2, 2mM CaCl2, 10% 1,5-hexanediol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Cacodylate | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | Spermidine | ||
| 5 | 1 | 2 | Cacodylate | ||
| 6 | 1 | 2 | CaCl2 | ||
| 7 | 1 | 2 | MgCl2 | ||
| 8 | 1 | 2 | 1,5-hexanediol | ||
| 9 | 1 | 2 | Spermidine |
