3G6L
The crystal structure of a chitinase CrChi1 from the nematophagous fungus Clonostachys rosea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.088, 71.715, 59.075 |
| Unit cell angles | 90.00, 91.25, 90.00 |
Refinement procedure
| Resolution | 45.590 - 1.800 |
| R-factor | 0.21237 |
| Rwork | 0.210 |
| R-free | 0.25739 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w9p |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.556 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.081 | 0.480 |
| Number of reflections | 30889 | |
| Completeness [%] | 95.0 | 92.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2M ammonium dihydrogen phosphate, 15%(w/v) PEG 3350, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
