3G5X
Antibodies Specifically Targeting a Locally Misfolded Region of Tumor Associated EGFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 140.372, 74.624, 83.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.22792 |
| Rwork | 0.225 |
| R-free | 0.28891 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.702 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.064 | 0.267 |
| Number of reflections | 44905 | |
| Completeness [%] | 98.3 | 91.3 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 293 | 0.1M sodium acetate, 6-8% PEG6000, 15-20% isopropanol, pH4.6, EVAPORATION, temperature 293K |
