3G5S

Crystal structure of Thermus thermophilus TrmFO in complex with glutathione

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.90000
Spacegroup name	P 21 21 21
Unit cell lengths	48.257, 92.226, 104.501
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.811 - 1.050
R-factor	0.163
Rwork	0.162
R-free	0.17840
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.179
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.850
High resolution limit [Å]	1.050
Number of reflections	209948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298	13% PEG 3350, 0.1M magnesium chloride, 0.05M Tris-HCl (pH 8.5), 10mM reduced-glutathione, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	8.5	298	13% PEG 3350, 0.1M magnesium chloride, 0.05M Tris-HCl (pH 8.5), 10mM reduced-glutathione, VAPOR DIFFUSION, temperature 298K