3G5L
Crystal structure of putative S-adenosylmethionine dependent methyltransferase from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-09-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.812, 92.532, 99.973 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.19235 |
| Rwork | 0.190 |
| R-free | 0.27618 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.199 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.121 | 0.430 |
| Number of reflections | 22700 | |
| <I/σ(I)> | 5.6 | 2.7 |
| Completeness [%] | 90.7 | 99.8 |
| Redundancy | 6.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 1M Lithium chloride pH 7.5, 100mM Sodium acetate, 30% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
