3G5K
Structure and activity of human mitochondrial peptide deformylase, a novel cancer target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.144, 77.830, 110.532 |
| Unit cell angles | 90.00, 107.46, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.700 |
| R-factor | 0.16504 |
| Rwork | 0.164 |
| R-free | 0.19347 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zxz |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.472 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 100609 | |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 1 M AMMONIUM PHOSPHATE MONOBASIC, 0.1 M SODIUM CITRATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
