3G4H
Crystal structure of Human Senescence Marker Protein-30 (Zinc Bound)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-16 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.365, 52.019, 85.832 |
| Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
| Resolution | 84.520 - 1.920 |
| R-factor | 0.22519 |
| Rwork | 0.224 |
| R-free | 0.25336 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g4e |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.582 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.520 | 2.020 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.086 | 0.013 |
| Number of reflections | 42805 | |
| <I/σ(I)> | 5.251 | 0.5 |
| Completeness [%] | 99.0 | 94 |
| Redundancy | 7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 298 | 38% PEG 6000, 0.1M Tris-Hcl, 5mM DTT, 5mM CaCl2, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
