3G48
Crystal structure of chaperone CsaA form Bacillus anthracis str. Ames
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2008-12-17 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.359, 67.564, 74.642 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.820 - 1.500 |
| R-factor | 0.16744 |
| Rwork | 0.166 |
| R-free | 0.20057 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nzh |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.745 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.077 | 0.560 |
| Number of reflections | 32247 | |
| <I/σ(I)> | 20 | 2.1 |
| Completeness [%] | 98.8 | 86.7 |
| Redundancy | 4.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 12% Peg 20000, 10 % glycerol, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP |
