3G3T
Crystal structure of a eukaryotic polyphosphate polymerase in complex with orthophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 136.110, 136.110, 84.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.647 - 1.850 |
| R-factor | 0.1964 |
| Rwork | 0.195 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g3q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 39665 | |
| <I/σ(I)> | 28.1 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.9 | 20.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | 1 M Na+/K+ phosphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
