3G3A
Structure of a lamprey variable lymphocyte receptor in complex with a protein antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.299, 133.651, 90.439 |
| Unit cell angles | 90.00, 114.69, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.22211 |
| Rwork | 0.220 |
| R-free | 0.27068 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g39 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.755 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.117 | 0.252 |
| Number of reflections | 88576 | |
| <I/σ(I)> | 12.6 | 3.2 |
| Redundancy | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 296 | 2.0 M ammonium sulfate and 0.1 M sodium citrate, pH 5.6, EVAPORATION, temperature 296K |
