3G2W
VHS Domain of human GGA1 complexed with a DXXLL hinge peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-04-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.044 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.800, 72.900, 103.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.289 - 2.400 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jwf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.431 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.544 |
| Number of reflections | 14702 | |
| <I/σ(I)> | 15.8 | 4.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.09 | 10.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 15%(w/v) PEG 5000 mmE, 0.2M NH4I, 0.3M 1,6-hexanediol, 0.1M MES-NaOH, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
