3G1Z
Structure of IDP01693/yjeA, a potential t-RNA synthetase from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-26 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.901, 68.905, 73.270 |
| Unit cell angles | 90.00, 110.31, 90.00 |
Refinement procedure
| Resolution | 47.510 - 1.950 |
| R-factor | 0.1841 |
| Rwork | 0.181 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Swiss-model (http://swissmodel.expasy.org/SWISS-MODEL.html) model using the input protein sequence |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.436 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.146 | 0.561 |
| Number of reflections | 54044 | |
| <I/σ(I)> | 20.05 | 3.82 |
| Completeness [%] | 96.1 | 85.8 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 1.6 M Sodium/Potassium dihydrogen phosphate, 0.1 M HEPES, pH 7.5, 0.5 mM ATP and 0.3 mM MgCl2, cryoprotected in N-Paratone oil , VAPOR DIFFUSION, HANGING DROP, temperature 295K |
