3G19
The structure of the Caulobacter crescentus clpS protease adaptor protein in complex with LLL tripeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.658, 34.419, 71.722 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.388 - 1.849 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.590 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.043 | 0.028 | 0.116 |
| Rmeas | 0.043 | ||
| Total number of observations | 33923 | ||
| Number of reflections | 5661 | ||
| <I/σ(I)> | 16.2 | ||
| Completeness [%] | 87.1 | 99.3 | 51.4 |
| Redundancy | 6 | 6.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 300 | 0.1 M bis-tris pH 5.5, 0.2 M MgCl2, 25% PEG 3350, vapor diffusion, temperature 300K |
