3G0L
Crystal Structure of Human Bromodomain Adjacent to Zinc finger domain 2B (BAZ2B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.434, 96.314, 57.641 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.220 - 2.030 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ensemble of 2NXB 2oo1 2oss 2ouo 2rfj 3dai |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.350 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.2) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.620 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.079 | 0.606 |
| Number of reflections | 14561 | |
| <I/σ(I)> | 12.5 | 2 |
| Completeness [%] | 96.4 | 94.4 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.1M MMT pH 6.0, 30% PEG 1000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
