3G0H
Human dead-box RNA helicase DDX19, in complex with an ATP-analogue and RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-20 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.570, 81.080, 124.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.620 - 2.700 |
| R-factor | 0.22014 |
| Rwork | 0.217 |
| R-free | 0.27520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ews |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.129 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.145 | 0.550 |
| Number of reflections | 12073 | |
| <I/σ(I)> | 9.1 | 2.5 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 277 | 14% PEGMME 2000, 0.25M trimethylamine n-oxide, 0.1 M Tris, pH 8, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEGMME 2000 | ||
| 2 | 1 | 1 | trimethylamine n-oxide | ||
| 3 | 1 | 1 | Tris | ||
| 4 | 1 | 2 | PEGMME 2000 | ||
| 5 | 1 | 2 | trimethylamine n-oxide | ||
| 6 | 1 | 2 | Tris |
