3FYD
Crystal Structure of Dim1 from the thermophilic archeon, Methanocaldococcus jannaschi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.236, 65.906, 61.932 |
| Unit cell angles | 90.00, 108.10, 90.00 |
Refinement procedure
| Resolution | 22.000 - 1.750 |
| R-factor | 0.18126 |
| Rwork | 0.176 |
| R-free | 0.22643 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | composite model consisting of monomers from pdb entries 1QYR 2H1R and 1ZQ9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.133 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | PHENIX (+ REFMAC 5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.100 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.038 | 0.294 |
| Number of reflections | 29365 | |
| <I/σ(I)> | 29.1 | 4.9 |
| Completeness [%] | 94.4 | 91.1 |
| Redundancy | 7.06 | 6.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 8000 (14-16%), 25 mM MES, 50 MM NH2SO4, 7 mM MgCl2, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
