3FXT
Crystal structure of the N-terminal domain of human NUDT6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-21 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 185.800, 185.800, 129.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.880 - 2.300 |
| R-factor | 0.20286 |
| Rwork | 0.201 |
| R-free | 0.23539 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.022 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.360 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.091 | 0.757 |
| Number of reflections | 58279 | |
| <I/σ(I)> | 17.09 | 3.08 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 7.05 | 6.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 19% PEG2000 MME, 0.1M Tris-HCl, 0.25M Trimethylamine. Chymotrypsin was added prior to crystallization to a ratio protease:protein of 1:100., pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
