3FX7
Crystal structure of hypothetical protein of HP0062 from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.23985 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.303, 51.895, 83.305 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.650 |
| R-factor | 0.18828 |
| Rwork | 0.186 |
| R-free | 0.22512 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gts |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.125 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.049 | 0.248 |
| Number of reflections | 20637 | |
| <I/σ(I)> | 59.33 | 10.97 |
| Completeness [%] | 97.1 | 95.1 |
| Redundancy | 12.8 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 5% PEG 3350, 100mM citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
