3FWU
Crystal structure of Leishmania major MIF1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2006-06-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97650 |
Spacegroup name | H 3 |
Unit cell lengths | 52.321, 52.321, 97.940 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.540 - 1.800 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uiz |
RMSD bond length | 0.008 |
RMSD bond angle | 1.185 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.069 | 0.121 |
Number of reflections | 9160 | |
<I/σ(I)> | 7.2 | |
Completeness [%] | 98.8 | 93.4 |
Redundancy | 5.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 30% w/v PEG 4000, 100mM Imidazole, pH 6.5, Hanging drop, temperature 290K, VAPOR DIFFUSION, HANGING DROP |