3FWJ
Ferric camphor bound Cytochrome P450cam containing a selenocysteine as the 5th heme ligand, orthorombic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.99187 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.100, 65.000, 105.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.380 - 1.900 |
R-factor | 0.185 |
Rwork | 0.183 |
R-free | 0.23100 |
Starting model (for MR) | 1akd |
RMSD bond length | 0.013 |
RMSD bond angle | 1.499 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.380 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.047 | 0.343 |
Number of reflections | 34678 | 4676 |
<I/σ(I)> | 3.4 | |
Completeness [%] | 99.0 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 24% PEG 4000, 50 mM Tris HCl, 250 mM KCl, 20 % glycerol for cryoprotection, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |