3FWG

Ferric camphor bound Cytochrome P450cam, Arg365Leu, Glu366Gln, monoclinic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-09-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9994
Spacegroup name	P 1 21 1
Unit cell lengths	67.180, 62.795, 95.605
Unit cell angles	90.00, 90.53, 90.00

Refinement procedure

Resolution	39.130 - 1.550
R-factor	0.186
Rwork	0.185
R-free	0.21700
RMSD bond length	0.009
RMSD bond angle	1.367
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.130	1.650
High resolution limit [Å]	1.550	1.550
Rmerge	0.056	0.347
Number of reflections	111321	17853
<I/σ(I)>		3.8
Completeness [%]	96.4	90.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	24% PEG 4000, 50 mM Tris HCl, 250 mM KCl, 20% glycerol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K