3FWE
Crystal Structure of the Apo D138L CAP mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Detector technology | CCD |
Collection date | 2007-11-11 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.996, 67.996, 230.113 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.650 - 2.300 |
R-factor | 0.23 |
Rwork | 0.227 |
R-free | 0.27700 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.106 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.940 | 2.357 |
High resolution limit [Å] | 2.300 | 2.297 |
Rmerge | 0.081 | 0.417 |
Number of reflections | 24422 | |
<I/σ(I)> | 7.199 | |
Completeness [%] | 67.1 | 64.8 |
Redundancy | 1.8 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 4000 and 200mM Proline, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |