3FVO
Crystal structure of the human glutamate receptor, GluR5, ligand-binding core in complex with 8-epi-neodysiherbaine A in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-10-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.717, 50.756, 63.290 |
| Unit cell angles | 94.19, 95.90, 117.95 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.16619 |
| Rwork | 0.165 |
| R-free | 0.19119 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2znt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.255 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 79930 | |
| <I/σ(I)> | 29.3 | 5.11 |
| Completeness [%] | 96.9 | 95.1 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 303 | 35% PEG3350, 0.3M LiSO4, 5mM 8-epi-neodysiherbaine, pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
